Content (Syllabus outline)
- Introduction: Microbiology as a basic and applied scientific discipline.
- Milestones in the development of microbiology. General role of microorganisms.
- Diversity, phylogenetics and taxonomy.
- Procaryotic and eucaryotic cells.
- Structure and function of bacteria, yeasts, phylamentous fungi, viruses and other microorganisms.
- Nutrition and metabolism of microbial cells. Nutrients and nutritional types of microorganisms. Catabolic and anabolic processes in microbial cells. Role of the main metabolic pathways – glycolysis, Krebs cycle, transport chain of electrones. Substrate phosphorylation and oxidative phosphorylation, different fermentation types, respiration, anaerobic respiration – diversity of metabolic processes in microbes. Energetics and mass balance of metabolic processes. Anabolic reactions – synthesis of microbial biomass.
- Growth of microorganisms. Theoretical basics of batch and continuous microbial growth. Growth curve, calculation of basic kinetic parameters of microbial growth. The influence of environmental factors on microbial growth. Measurement of microbial growth. Kinetics of microbial inactivation.
- Basics of genetics and molecular genetics of microorganisms. Nucleic acids structure and experimental techniques for studying nucleic acids structure. Replication of microbial DNA. Genetic elements and transfer of genetic information from DNA to proteins. Methods in microbial and molecular genetics. Regulation of gene expression – specific and global regulation mechanisms (principles of positive and negative regulation, catabolic repression etc.)
- Microbial genetics – mutations and recombinations.
Prerequisites
Condition for laboratory work is inscription in the study year and knowledge of theoretical principles and practical tips for work (the script).
Conditions for carrying out colloquium are performed laboratory exercises and reviewed laboratory diary, conditions for carrying out the exam is passed colloquium.